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| Catalog Code: | NB110-55300 |
| Product Name: | STIM1 Antibody |
| Product Description: | Mouse Monoclonal anti-STIM1 (CDN3H4) |
| Clone: | CDN3H4 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetized peptide (C-terminal intracelular part of STIM1). |
| Specificity: | The antibody CDN3H4 reacts with mouse and rat STIM1, a 84 kDa essential and conserved component of store-operated Ca2+ channel function. |
| Cross Reactivity: | Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | Western Blot can be used in both reducing and non-reducing conditions. |
| Background: | STIM1 (Stromal Interaction Molecule 1; GOK) is an essential and conserved component of store-operated Ca2+ channel function. It is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane (STIM1 translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium). STIM1 is a typical transmembrane protein with an extracellular N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that likely represents the transmembrane region. The mouse STIM1 homologue (Stim1) was identified independently as a stromal-cell-derived gene product that binds to the surface of haematopoietic cells, particularly pre-B lymphoid cells, and can promote subsequent clonal expansion. Murine Stim1 is 96% identical with human STIM1 in amino acid sequence, and maps to a region of mouse chromosome 7 syntenic with human 11p15.5. |
| Storage: | Store at 4 degrees C short term and -20 degrees C long term. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Phosphate buffered saline (PBS), approx. pH 7.4 |
| Application Summary: | Western Blot |
| Species Summary: | Mu, Rt, |
| Package Size: | 0.1 mg |
| Preservative: | 15 mM sodium azide |
| General References: | *Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA.: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85. *Draber P, Draberova L.: Lifting the fog in store-operated Ca2+ entry. Trends Immunol. 2005 Dec;26(12):621-4. |
| Novus References: | 1. Prakash YS, Thompson MA, Vaa B, et al. Caveolins and intracellular calcium regulation in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2007 November 1, 2007;293(5):L1118-26. |
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