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| Catalog Code: | NB110-53818 |
| Product Name: | ATG5 Antibody |
| Product Description: | Rabbit Polyclonal anti-ATG5 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1.17 mg/ml |
| Immunogen: | A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. [Swiss-Prot# Q9H1Y0] |
| Specificity: | This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275. |
| Cross Reactivity: | This antibody reacts with the mouse form of the protein. Other species have not been tested. |
| Packaging: | 0.1 ml peptide affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western blot analysis. The molecular weight of human ATG5 is ~33 kDa. |
| Localization: | Cytoplasm. Co-localizes with non-muscle actin. |
| Background: | Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | peptide affinity purified |
| Host Name: | Rabbit |
| Buffer: | PBS plus 30% glycerol |
| Application Summary: | immunohistochemistry 2.5ug/ml, Western Blot 1:500 |
| Species Summary: | Mu |
| Alternate Names: | anti-Autophagy protein 5 antibody; anti-APG5-like antibody; anti-Apoptosis-specific protein antibody; anti-APG5L antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% sodium azide |
| Homology: | 100% with human, mouse, rat, cow, pig, monkey, and Xenopus. 94% with Zebrafish. |
| General References: | 1. Mizushima, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell. Biol. 2001. 152: 657-668 |
| Novus References: | 1. Wang, Y., R. Singh, et al. Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus. J. Biol. Chem. 2008; 283: 4766-4777. (Immunohistochemistry) 2. Pyo, J., Nah, J., Kim, H., et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy. April 2008; 4:3, 315-321. |
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