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| Catalog Code: | NB110-4907 |
| Product Name: | Progesterone Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Progesterone Receptor (PR-AT 4.14) |
| Clone: | PR-AT 4.14 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: (533)RPGEAAVTAAPASAS(547). Immunizing peptide corresponds to amino acid residues 533-547 of the human progesterone receptor DNA binding domain. |
| Specificity: | Detects progesterone receptor. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor. |
| Cross Reactivity: | Cross-reacts with Human, Rabbit, Rat, Mouse and Cow. Not yet tested in other species. |
| Packaging: | 0.05 mg Mouse ascites. |
| Uses: | Immunohistochemistry: use at a concentration of 5 ug/ml. Western Blot: use at a concentration of 5 ug/ml. Immunohistochemistry: Assay dependent. Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from rat uterine homogenate. Immunohistochemical staining of PR in rat uterus with this antibody yields intense nuclear staining. |
| Localization: | Nuclear |
| Background: | The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC 1) or corepressor (e.g. NCoR) proteins. PgR is phosphorylated on multiple serine residues through distinct groups of sites regulated by hormone and different kinases. Progesterone receptor is present in the CNS in monoenzymatic aromatic L amino acid decarboxylase (AADC) containing cells of the preoptic area and also in neurons expressing both AADC and tyrosine hydroxylase (TH) in the arcuate nucleus. It has been proposed that expression of PgR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PgR determination provides supplementary information to ER, both in predicting response to endocrine therapy and estimating survival. PgR has proved superior to ER as a prognostic indicator in some studies. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry-Fr, immunoprecipitation, Western Blot |
| Species Summary: | Bv, Hu, Mu, Rt, Rb, |
| Alternate Names: | anti-NR3C3 antibody, anti-PGR antibody, anti-PR antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.1% sodium azide |
| General References: | This product has been used in:Ohta Y et al. Effect of estrogen on ontogenic expression of progesterone and estrogen receptors in rat uterus. Zoolog Sci 13:143-9 (1996). Ohta Y Sterility in neonatally androgenized female rats and the decidual cell reaction. Int Rev Cytol 160:1-52 (1995). Ohta Y et al. Development and mitotic activity of the metrial gland in rat uterus during mid-pregnancy. In Vivo 7:121-6 (1993). Ohta Y et al. Immunocytochemical localization of progesterone receptor in the reproductive tract of adult female rats. Biol Reprod 48:205-13 (1993). Traish AM & Wotiz HH Monoclonal and polyclonal antibodies to human progesterone receptor peptide-(533-547) recognize a specific site in unactivated (8S) and activated (4S) progesterone receptor and distinguish between intact and proteolyzed receptors. Endocrinology 127:1167-75 (1990). General / background references:Zheng J et al. Estrogen and progesterone receptors, cell proliferation, and c-fos expression in the ovine uterus during early pregnancy. Endocrinology 137:340-8 (1996). |
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