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| Catalog Code: | NB110-4797 |
| Product Name: | IL-1 Receptor Antagonist Antibody |
| Product Description: | Rabbit Polyclonal anti-IL-1 Receptor Antagonist |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant human IL-1Ra produced in E.coli. |
| Specificity: | This antibody does not recognize human IL-1 alpha , IL-1 beta or IL-1 receptor type I or type II. It is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). |
| Cross Reactivity: | Cross-reacts with Primate. Does not cross-react with Rabbit or Mouse. Not yet tested in other species. |
| Packaging: | 0.5 mg IgG purified Rabbit antisera. |
| Uses: | Western blot: 1:400 - 800 dilution:When used in immunoblot analysis, this antibody will recognize 17kDa IL-1Ra released from supernatants or lysates of 2 x 10<sup>6</sup> endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly. Neutralisation: 1:200 dilution:This antibody is also useful for neutralization of human and primate IL-1Ra. If a body fluid, cell supernatant or serum contains IL-1Ra, it is possible to neutralize the inhibitory activity of IL-1Ra on IL-1 activity, being exogenous or endogenous IL-1. A 1:200 dilution of this antibody is incubated with the sample for 4 hours. The mixture is then added to cells in the assay for IL-1 activity. A control of normal rabbit IgG is recommended. An increase in the activity of the sample indicates that the inhibitory activity of IL-1Ra on an IL-1-mediated read-out has been neutralized. Using recombinant human IL-1Ra as a positive control, a 1:200 dilution of this antibody will neutralize the inhibitory activity of IL-1Ra at 100 ng/mL. This antibody does not neutralize the biological activity of IL-1. ELISA: 1:500 - 1000 dilution:For use in ELISA, this antibody is best used as the secondary antibody in combination with a monoclonal antibody as a capture antibodyIHC: 1:400 dilutionRIA: 1:8000 dilutionFACS: Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.Optimal dilutions should be determined by the investigator. |
| Background: | IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 0.02M K Phosphate, 0.15M NaCl, pH7.2 |
| Application Summary: | flow cytometry / FACS analysis , ELISA , immunohistochemistry -P, Western Blot , Radioimmunoassay |
| Species Summary: | Mu(-), Mk, Rb(-), |
| Alternate Names: | anti-ICIL 1RA antibody, anti-IL1 inhibitor antibody, anti-ICIL1RA antibody, anti-IL1F3 antibody, anti-IL1RN antibody, anti-Interleukin 1 Receptor Antagonist Protein antibody, anti-IRAP antibody |
| Package Size: | 0.5 mg |
| Preservative: | 0.01% Sodium Azide |
| General References: | General / background references:Arend WP et al. Interleukin-1 receptor antagonist: role in biology. Annu Rev Immunol 16:27-55 (1998). Hammerberg C et al. Interleukin-1 receptor antagonist in normal and psoriatic epidermis. J Clin Invest 90:571-83 (1992). Haskill S et al. cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium. Proc Natl Acad Sci U S A 88:3681-5 (1991). Eisenberg SP et al. Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist. Nature 343:341-6 (1990). |
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