| CatalogCode: | | NB110-39113 |
| ProductName: | | GLUT1 Antibody |
| Product Description: | | Rabbit Polyclonal anti-GLUT1 |
| Clonality: | | Polyclonal |
| Immunogen: | | A synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot #P11166] |
| Specificity: | | This antibody is specific for GLUT1 protein. |
| CrossReactivity: | | This antibody is reactive against the human protein. No other specie has been tested. |
| Packaging: | | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | | By Western blot a band is seen at ~42 kDa on human kidney membrane prep, representing GLUT1 protein. We have not been successful detecting the band in anything other than membrane preps. Depending on the tissue and any post-translational modifications, this protein can run anywhere betweeen 40-60 kDa. Boiling samples prior to gel loading will often result in the protein running as a smear, rather than a distinct band. |
| Localization: | | Cell membrane |
| Control: | | Human kidney membrane and human skeletal muscle. |
| Background: | | Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are seven types of glucose transport carrier proteins, designated as Glut 1 to 7. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes, as in blood brain barrier and blood testis barriers. GLUT1 is a major glucose transporter in the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells. |
| ProductRef: | | 1. Hergovich, A., Lisztwan, J., Thoma, C. R., Wirbelauer, C., Barry, R. E., and Krek, W. (2006) Priming-Dependent Phosphorylation and Regulation of the Tumor Suppressor pVHL by Glycogen Synthase Kinase 3, 26, 5784-5796. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Host_Name: | | Rabbit |
| Buffer: | | Tris-glycine, 150mM NaCl |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Erythrocyte/brain HepG2 glucose transporter antibody; anti-Glucose transporter type 1 antibody; anti-Glucose transporter type 1 erythrocyte/brain antibody; anti-GLUT antibody; anti-GLUTB antibody; anti-GT1 antibody; anti-GTG1 antibody; anti-Gtg3 antibody; anti-SLC2A1 antibody; anti-Solute carrier family 2 facilitated glucose transporter member 1 antibody |
| PackageSize: | | 0.1 ml |
| Homology: | | 100% human and 93% mouse, rat, cow, and rabbit |
| more information: | | company product webpage |