| CatalogCode: | NB110-3158 |
| ProductName: | PCNA Antibody |
| Product Description: | Mouse Monoclonal anti-PCNA (PC10), conjugated to FITC |
| Clone: | PC10 |
| Clonality: | Monoclonal |
| Immunogen: | Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T |
| Specificity: | This antibody reacts with a nuclear antigen that is expressed by proliferating cells in all phases of the active cell cycle from all vertebrate species and insects. |
| CrossReactivity: | Cross-reacts with Human, Drosophilia melanogaster, Mouse, Rat, S. cerevisiae and S. pombe. Not yet tested in other species. |
| Packaging: | 50 tests Immunogen affinity purified Mouse ascites. |
| Uses: | This antibody can be used to immunostain malignant tumours with low and high proliferation on paraffin wax embedded sections This antibody can be used to study cell proliferation with PHA stimulated peripheral blood lymphocytes and various neoplasms. For flow cytometry we recommend using 20ul of conjugate per million cells or 100ul of whole blood. |
| Localization: | Nuclear |
| Background: | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | PBS, 1% BSA |
| ListPrice: | 295 |
| AppSummary: | FACS, IHC-P |
| SpeciesSummary: | Dr, Hu, Mu, Rt, Ye |
| ALTnames: | anti-Cyclin antibody, anti-DNA polymerase delta auxiliary protein antibody, anti-HGCN8729 antibody, anti-MGC8367 antibody, anti-polymerase delta accessory protein antibody, anti-Proliferating Cell Nuclear Antigen antibody |
| ProteinTarget: | PCNA |
| PackageSize: | 50 tests |
| Conjugate: | FITC |
| GeneralRef: | This product has been used in:Hall PA et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285-94 (1990). General / background references:Coltrera MD & Gown AM PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines. J Histochem Cytochem 39:23-30 (1991). Waseem NH & Lane DP Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci 96 ( Pt 1):121-9 (1990). Robbins BA et al. Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies. Arch Pathol Lab Med 111:841-5 (1987). Kurki P et al. Proliferating cell nuclear antigen (PCNA)/cyclin in activated human T lymphocytes. J Immunol 138:4114-20 (1987). Celis JE et al. Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells. Leuk Res 8:143-57 (1984). |