| CatalogCode: | NB110-2546 |
| ProductName: | Troponin I Antibody |
| Product Description: | Mouse Monoclonal anti-Troponin I (19C7) |
| Clone: | 19C7 |
| Clonality: | Monoclonal |
| Immunogen: | Native troponin complex |
| Epitope: | 41-49 aa |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Cat, Dog, Fish, Goat, Pig and Rabbit. Not yet tested in other species. |
| Packaging: | 0.2 mg protein A purified Mouse ascites. |
| Uses: | AP: Use at an assay dependent concentration. ELISA: Use at an assay dependent dilution.IA: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. These antibody combinations are recommended for sandwich ELISA, and demonstrate high sensitivity (0.02 ng/ml), good kinetics, low background and high reproducibility: detection. Using multiple combinations of MAbs antibodies with different epitope specificity as capture and multiple MAbs as detection, the sensitivity of the sandwich ELISA can be increased 2-4 fold. The ELISA based on these recommended Abs, recognize complexed and free forms of the antigen with same sensitivity. For better sensitivity and reproducibility for assay designing we recommend to use at least one antibody that recognizes the stable fragment of the cTnI molecule (30-110 aa section), as cTnI is proteolytically degraded in the blood stream.Because the amount of complexed cTnI in blood of AMI patients is very high, for cTnI immunodetection we recommend using also a MAb that is specific to TnC or a MAb specific to TnC together with MAb(s) specific to cTnI. Such approach helps improving the sensitivity and reproducibility of cTnI immunoassays. |
| Background: | Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | PBS with pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | ELISA, IP, WB, IA, AP |
| SpeciesSummary: | Bv, Ca, Hu, Mu, Po, Rt, Rb, Fe, Fi, Gt |
| ProteinTarget: | Troponin I |
| PackageSize: | 0.2 mg |
| GeneralRef: | General / background references:Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). Cummins B et al. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:1333-44 (1987). |
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