| CatalogCode: | NB110-2545 |
| ProductName: | Troponin I Antibody |
| Product Description: | Mouse Monoclonal anti-Troponin I (414) |
| Clone: | 414 |
| Clonality: | Monoclonal |
| Immunogen: | Free human cardiac troponin I |
| Epitope: | 56-61 aa |
| Specificity: | Reacts only with free troponin INot affected by heparin, phosphorylation, oxidation and troponin complex formation. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Cow, Dog, Fish, Pig and Rabbit.Does not cross-react with Rat, Cat or Goat.Not yet tested in other species. |
| Packaging: | 0.2 mg |
| Uses: | ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. Can be paired for ELISA with <ab10232>Mouse monoclonal [8E10] to cardiac Troponin I</ab> and <ab10222>Mouse monoclonal [7F4] to cardiac Troponin T</ab>. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | MAb 414 reacts only with the free form of cTnI and does not recognize cTnI in complex with TnC. The epitope of this MAb is blocked or changed by TnC in binary TnI - TnC complex. Thus this monoclonal antibody can be used only for the immunodetection of the free form of the antigen. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS with pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | EIA, ELISA, WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | Troponin I |
| PackageSize: | 0.2 mg |
| GeneralRef: | General / background references:Marston SB & Redwood CS Modulation of thin filament activation by breakdown or isoform switching of thin filament proteins: physiological and pathological implications. Circ Res 93:1170-8 (2003). Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). Onuoha GN et al. Skeletal troponin-I release in orthopedic and soft tissue injuries. J Orthop Sci 6:11-5 (2001). Bertinchant JP et al. Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats. Clin Chim Acta 298:13-28 (2000). Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). Reiffert SU et al. Stepwise subunit interaction changes by mono- and bisphosphorylation of cardiac troponin I. Biochemistry 37:13516-25 (1998). Katrukha AG et al. Degradation of cardiac troponin I: implication for reliable immunodetection. Clin Chem 44:2433-40 (1998). Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). Jaquet K et al. Pattern formation on cardiac troponin I by consecutive phosphorylation and dephosphorylation. Eur J Biochem 231:486-90 (1995). Katus HA et al. Enzyme linked immuno assay of cardiac troponin T for the detection of acute myocardial infarction in patients. J Mol Cell Cardiol 21:1349-53 (1989). Cummins B et al. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:1333-44 (1987). |
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