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| Catalog Code: | NB110-1922 |
| Product Name: | Fibronectin Antibody |
| Product Description: | Rabbit Polyclonal anti-Fibronectin, conjugated to Biotin |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE |
| Specificity: | negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible. |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with most Mammals due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Whole Rabbit antisera. |
| Localization: | Extracellular matrix. |
| Background: | This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 15mg/ml BSA |
| Application Summary: | ELISA 1/4000-1/8000, immunoprecipitation , Western Blot 1/5000-1/10000, Immunohistochemistry-Paraffin 1/50-1/200 |
| Species Summary: | Hu, |
| Alternate Names: | Anti-CIG antibody; Anti-Cold insoluble globulin antibody; Anti-DKFZp686F10164 antibody; Anti-DKFZp686H0342 antibody; Anti-DKFZp686I1370 antibody; Anti-DKFZp686O13149 antibody; Anti-fibronectin 1 antibody; Anti-FINC antibody; Anti-FN 1 antibody; Anti-FN antibody; Anti-FN1 antibody; Anti-LETS antibody; Anti-MSF antibody; Anti-Transformation sensitive protein antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Na azide, 0.01% Thimerisol |
| Conjugate: | Biotin |
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more information: | company product webpage |