| CatalogCode: | NB110-1244 |
| ProductName: | MDA 5 Antibody |
| Product Description: | Goat Polyclonal anti-MDA 5 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: SNGYSTDENFRYL representing the N-Terminus of the human protein (residues 2-14), according to NP_071451. |
| CrossReactivity: | Reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: No signal obtained yet but low background observed in Human Placenta lysate at up to 1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10ug/ml.Recommended Starting Dilution:*ELISA (peptide): 1/32000WB: unknownIHC: 10ug/ml*Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, IHC, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide antibody, anti-Melanoma Differentiation Associated Protein 5 antibody, anti-IFIH1 antibody, anti-interferon induced with helicase C domain 1 antibody, anti-MDA5 antibody, anti-melanoma differentiation associated protein-5 antibody, anti-DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide antibody |
| ProteinTarget: | MDA 5 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Kang DC et al. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc Natl Acad Sci U S A 99:637-42 (2002). |
| NotesMain: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. |
| Gene_Id: | 64135 |
| Omim_ID: | NB110-67834 |
|