| CatalogCode: | NB110-11450 |
| ProductName: | TCP1 beta Antibody |
| Product Description: | Rat Monoclonal anti-TCP1 beta (PK/8/4/4i/2f) |
| Clone: | PK/8/4/4i/2f |
| Clonality: | Monoclonal |
| Immunogen: | TCP1 beta peptide conjugated to purified tuberculin protein derivative (PPD) (Mouse). |
| Specificity: | This antibody does not react with human Hsp60 nor with E. coli GroEL proteins. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Chicken, Dog, Hamster, Monkey, Pig, Rabbit and Sheep. Not yet tested in other species. |
| Packaging: | 0.05 mg Affinity purified Rat ascites. |
| Uses: | WB: Use at a concentration of 1 ug/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa). |
| Localization: | Cytoplasmic |
| Background: | TCP1 beta is a subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. These chaperonins assist the folding of proteins upon ATP hydrolysis. Nine different subunits of TCP-1 containing chaperonin complexes from mammalian testis and seven different subunits of mouse F9 cells have been identified. It has been suggested that each CCT subunit has a specific, independent function, as they are highly diverged from each other but conserved from mammals to yeast. The expansion in the number of types of CCT subunit, compared with other chaperonins, has allowed CCT to carry out more complex functions that are required for the folding and assembly of highly evolved eukaryotic proteins. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG2b |
| Host_Name: | Rat |
| Buffer: | Constituents: PBS, 50mM Sodium Chloride, 10mM Sodium Phosphate, 0.1mM PMSF. pH 7.2 |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Bv, Ca, Ha, Hu, Mu, Rt, Rb, Sh |
| ALTnames: | anti-CCT2 antibody, anti-CCTB antibody, anti-Chaperonin containing t-complex polypeptide 1 beta subunit antibody, anti-Chaperonin containing TCP1 subunit 2 (beta) antibody, anti-T-complex protein 1 beta subunit antibody |
| ProteinTarget: | TCP1 beta |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Hynes G et al. Antibody characterisation of two distinct conformations of the chaperonin-containing TCP-1 from mouse testis. FEBS Lett 358:129-32 (1995). Kubota H et al. Identification of six Tcp-1-related genes encoding divergent subunits of the TCP-1-containing chaperonin. Curr Biol 4:89-99 (1994). Willison K et al. The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes. Cell 57:621-32 (1989). Willison KR et al. Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell 44:727-38 (1986). |
| NotesMain: | For maximum product recovery, after thawing, centrifuge the product vial before removing cap. |
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