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| Catalog Code: | NB110-11069 |
| Product Name: | Agrin Antibody |
| Product Description: | Mouse Monoclonal anti-Agrin (AGR 131) |
| Clone: | AGR 131 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant rat agrin, C-terminal construct. |
| Epitope: | Epitope is located near the 1st EGF-like repeat. |
| Specificity: | Ab12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants. |
| Cross Reactivity: | Cross-reacts with Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 mg Mouse ascites. |
| Uses: | WB: Predicted molecular weight: 208.6 kDa. |
| Localization: | Synaptic basal lamina at the neuromuscular junction. |
| Background: | Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
| Application Summary: | immunohistochemistry , Western Blot 2 ug/ml, Immunocytochemistry 1/50 - 1/1000, RID, |
| Species Summary: | Mu, Rt, |
| Alternate Names: | anti-AGRN antibody, anti-FLJ45064 antibody, anti-OTTHUMP00000044043 antibody |
| Package Size: | 0.1 mg |
| Preservative: | No Preservative |
| General References: | General / background references:Grow WA & Gordon H Sialic acid inhibits agrin signaling in C2 myotubes. Cell Tissue Res 299:273-9 (2000). Hoch W Formation of the neuromuscular junction. Agrin and its unusual receptors. Eur J Biochem 265:1-10 (1999). Groffen AJ et al. Agrin is a major heparan sulfate proteoglycan in the human glomerular basement membrane. J Histochem Cytochem 46:19-27 (1998). Sharp AA & Caldwell JH Aggregation of sodium channels induced by a postnatally upregulated isoform of agrin. J Neurosci 16:6775-83 (1996). Campanelli JT et al. Alternative RNA splicing that determines agrin activity regulates binding to heparin and alpha-dystroglycan. Development 122:1663-72 (1996). Glass DJ et al. Agrin acts via a MuSK receptor complex. Cell 85:513-23 (1996). Bowen DC et al. Neural agrin activates a high-affinity receptor in C2 muscle cells that is unresponsive to muscle agrin. J Neurosci 16:3791-7 (1996). Hoch W et al. Structural domains of agrin required for clustering of nicotinic acetylcholine receptors. EMBO J 13:2814-21 (1994). Ferns MJ et al. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11:491-502 (1993). Tsim KW et al. cDNA that encodes active agrin. Neuron 8:677-89 (1992). Wallace BG Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9:1294-302 (1989). |
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