| CatalogCode: | NB100-904 |
| ProductName: | 53BP1 Antibody |
| Product Description: | Rabbit Polyclonal anti-53BP1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158). This antibody is a cocktail hybrid of NB 100-304 and NB 100-305. |
| Specificity: | NB 100-904 is specific for human 53BP1 protein. |
| CrossReactivity: | NB 100-904 reacts with human in Western Blot and immunocytochemistry and in it reacts with mouse in immunocytochemistry (Western Blot has not been done using this antibody on mouse protein). Other species have not been tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | NB 100-904 may be used for Western Blot where a band at ~220 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. Irradiated lysates demonstrate a gel shift.Suggested working dilutions: *Western Blot - 1:1,000-1:10,000 (ECL)Immunocytochemistry - 1:100-1:1,000Immunoprecipitation - Not Done |
| Control: | U205 cell lysates |
| Background: | P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| ProductRef: | 1. Yanagawa, T., T. Funasaka, et al. (2007). Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Activities by the Poly(ADP-Ribose) Polymerase Family-14. 67: 8682-8689. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate (pH 7-8) buffer. |
| ListPrice: | 285 |
| NovusRef: | 1. Murga, M., I. Jaco, et al. (2007). Global chromatin compaction limits the strength of the DNA damage response. 178: 1101-1108. 2. Pennarun G, Granotier C, Hoffschir F, et al. Role of ATM in the telomere response to the G-quadruplex ligand 360A. Nucleic Acids Res. March 1, 2008;36(5):1741-54. 3. Toledo, L. I., M. Murga, et al. (2008). ATR signaling can drive cells into senescence in the absence of DNA breaks. Genes Dev. 22: 297-302. |
| AppSummary: | WB, ICC |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-53BP1 antibody, anti-53BP-1 antibody, anti-TP53BP1 antibody, anti-Tumor Protein p53 Binding Protein 1 antibody |
| ProteinTarget: | 53BP1 (p53 Binding Protein 1) antibody cocktail |
| PackageSize: | 0.1 ml |
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