| CatalogCode: | NB100-898 |
| ProductName: | SOCS4 Antibody |
| Product Description: | Goat Polyclonal anti-SOCS4 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide SKVRVLRIDAPEQQ, representing the C-terminus of the human protein (residues 426-439), according to NP_543143. |
| CrossReactivity: | Expected to cross-react with Human and cow due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments in Human and Rodent Brain lysates and in lysates of cell lines NIH3T3, A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Background: | Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes. SOCS6 and SOCS7 are expressed ubiquitously in murine tissues and SOCS6 knockout mice are growth retarded. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 195 |
| AppSummary: | ELISA, WB |
| ALTnames: | anti-SH2 domain containing SOCS box protein antibody, anti-SOCS4 antibody, anti-Suppressor of cytokine signaling 4 antibody |
| ProteinTarget: | SOCS4 antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | Kile BT et al. The SOCS box: a tale of destruction and degradation. Trends Biochem Sci 27:235-41 (2002). <PUBMED:12076535> |
| NotesMain: | Principal Names ? SOCS7; SOCS4; suppressor of cytokine signaling 7; SH2 domain containing SOCS box protein. Official Gene Symbol - SOCS7. GenBank Accession Number ? NP_543143. LocusLink ID - 122809 (human). Gene Ontology terms - intracellular signaling cascade; regulation of cell growth. |
| Gene_Id: | 122809 |
| Omim_ID: | H00122809-M01 |