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| Catalog Code: | NB100-897 |
| Product Name: | APE1 Antibody |
| Product Description: | Goat Polyclonal anti-APE1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Gene ID: | 328 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide with sequence PKRGKKGAVAEDGD, representing the N Terminus of the protein (residues 1-14) according to NP_001632; NP_542379; NP_542380. |
| Cross Reactivity: | Tested: Human Expected from sequence similarity: Human |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1. Recommended concentration: 0.5-2ug/ml.. |
| Background: | Apurinic / apyrimidinic endonuclease (APE1 or REF1) is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. Alterations in APE1 levels have been described in some cancers. APE1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| Application Summary: | ELISA 1:32000, Western Blot .5-2ug/ml |
| Species Summary: | Hu |
| Alternate Names: | anti-AP endonuclease class I antibody, anti-AP lyase antibody, anti-APEN antibody, anti-Apex Nuclease antibody, anti-APEX1 antibody, anti-Apurinic/apyrimidinic exonuclease antibody, anti-HAP1 antibody, anti-Redox Factor 1 antibody, anti-REF1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Na azide |
| General References: | Demple B et al. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc Natl Acad Sci U S A 88:11450-4 (1991). |
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