| CatalogCode: | NB100-893 |
| ProductName: | BAK Antibody |
| Product Description: | Goat Polyclonal anti-BAK |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide ASGQGPGPPRQE, representing the N-Terminus of the human protein (residues 2-13), according to NP_001179 |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Rat and Mouse due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for Western Blot, ? Approx 25kDa band seen in A431 cell lysate. Not tested in other applications.Recommended Starting Dilution:WB: 0.5-1.0ug/ml*Optimal dilutions should be determined by the end user. |
| Background: | Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of holologous proteins which function in the regulation of apoptosis. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relativeratios of the different Bcl2 family proteins. Bak is a 211 aa protein (24kD) which has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. Bak has been localized by immunohistochemistry to the cytosol of cells and stains in a punctate pattern characteristic of other bcl2 family proteins, which have been localized to intracellular organelles. Immunohistochemical studies further show that Bak expression is widespread, and may show some correlation with the apoptopic state of cells. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Apoptosis Regulator Bak antibody, anti-BAK antibody, anti-BAK1 antibody, anti-Bcl2 homologous antagonist killer antibody, anti-Bcl2 like 7 Protein antibody, anti-BCL2-antagonist/killer 1 antibody, anti-BCL2L7 antibody, anti-CDN1 antibody, anti-Cell death inhibitor 1 antibody, anti-NBak antibody |
| ProteinTarget: | Bak |
| PackageSize: | 0.1 mg |
| GeneralRef: | Derry JM et al. Isolation of a novel gene mutated in Wiskott-Aldrich syndrome. Cell 78:635-44 (1994). |
| Gene_Id: | 578 |
| Omim_ID: | NB120-2351 |
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