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| Catalog Code: | NB100-866 |
| Product Name: | GRB 2 Antibody |
| Product Description: | Goat Polyclonal anti-GRB 2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Gene ID: | 2885 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide with sequence PRNYVTPVNRNV, representing the C Terminus of the protein (residues 206-217) according to NP_002077. |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Rat and Mouse due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used in Western Blot where a 30kDa band is seen in Jurkat lysate and in immunohostochemistry on human tonsil paraffin sections, where it stains the mantle zone and to a lesser extent the germinal center. Recommended Starting Dilutions*:WB: 1.0-3.0 ug/mlIHC-P: 01-0.3 ug/ml*Optimal dilutions should be determined by the end user. |
| Localization: | Cytoplasmic (probable) |
| Background: | Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| Application Summary: | immunohistochemistry -P, Western Blot , Immunohistochemistry-Paraffin 0.3ug/ml |
| Species Summary: | Hu |
| Alternate Names: | anti-Abundant SRC homology antibody, anti-Ash protein antibody, anti-Epidermal growth factor receptor binding protein antibody, anti-GRB2 adapter protein antibody, anti-Growth Factor Receptor Bound Protein 2 antibody, anti-Growth factor receptor bound protein 3 antibody, anti-HT027 antibody, anti-SEM5 antibody, anti-SH2/SH3 adapter GRB2 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Na azide |
| General References: | Lowenstein EJ et al. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell 70:431-42 (1992). |
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