| CatalogCode: | NB100-835 |
| ProductName: | ATF2 Antibody |
| Product Description: | Goat Polyclonal anti-ATF2 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide with sequence IVMAPSSQSQPSGS representing the C-terminus of the human protein (residues 492-505), according to NP_001871. |
| CrossReactivity: | Cross-reacts with Human, Rat. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used in Western Blot where 60kDa and 28 kDa bands are seen in HeLa cell lysates. Please note that the appox 28KDa band we observe maybe explained by the isoform described in Bailey et al [J. Clin. Endocr. Metab. 87: 1717-1728, 2002].Recommended Starting Dilutions*:WB: 0.5-2ug/ml. Overnight incubation is recommended with this antibody. *Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear |
| Background: | ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-Activating Transcription Factor 2 antibody, anti-cAMP Response Element Binding Protein 2 antibody, anti-CRE BP1 antibody, anti-CREB 2 antibody, anti-CREB2 antibody, anti-CREBP1 antibody, anti-HB 16 antibody, anti-HB16 antibody, anti-MGC111558 antibody, anti-mXBP antibody, anti-TREB 7 antibody, anti-TREB7 antibody |
| ProteinTarget: | ATF 2 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Monzen K et al. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol 153:687-98 (2001). PUBMED:11352931 |
| Gene_Id: | 1386 |
| Omim_ID: | NB100-1876 |