| request information: |
|
| Catalog Code: | NB100-80035 |
| Product Name: | PRC1 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-PRC1 Phospho (EP1514Y) [Thr481] |
| Clone: | EP1514Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Reference Sequence: | O43663 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481. |
| Specificity: | A phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481. |
| Cross Reactivity: | This antibody is reactive against Human |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, immunohistochemistry, Immunocytochemistry |
| Background: | Novel human protein Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis (1). PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Overexpression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization. PRC1 is phosphorylated by Cdc2 at Thr470 and Thr481 during mitosis. (2). PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:500, immunohistochemistry 1:100-250, Immunocytochemistry 1:100-250 |
| Species Summary: | Hu |
| Alternate Names: | Anti-Protein Regulator of Cytokinesis 1 antibody |
| Package Size: | 0.1 ml |
| Phosphorylated: | Thr481 |
| General References: | 1. Jiang W, et al. Mol Cell 2(6):877-85, 1998. 2. Mollinari C, et al. J. Cell Biol 157(7):1175-86, 2002 3. Ban R, et al. J Biol Chem 279(16):16394-402, 2004. |
order or
more information: | company product webpage |