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| Catalog Code: | NB100-80031 |
| Product Name: | Pol II Antibody |
| Product Description: | Rabbit Monoclonal anti-Pol II (EPR1509Y) |
| Clone: | EPR1509Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Reference Sequence: | P24928 |
| Immunogen: | A synthetic peptide corresponding to residues near the N-terminus of human Pol II was used as an immunogen. |
| Specificity: | A synthetic peptide corresponding to residues near the N-terminus of human Pol II was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against Human |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, Immunocytochemistry, flow cytometry / FACS analysis |
| Background: | DNA-dependent RNA polymerase II (Pol II), a complex multisubunit enzyme, is responsible for the transcription of protein-coding genes. This complex interacts with the promoter regions of genes as well as with a variety of elements and transcription factors to determine essentially all of the parameters that govern transcription. A fraction of the large subunit of Pol II is ubiquitinated after exposing cells to UV radiation or cisplatin suggesting that ubiquitination of Pol II plays a role in the recognition and/or repair of damage to actively transcribed genes (1). Evidence demonstrates that pol II transcription termination involves two distinct and temporally separate events. The first event, termed pretermination cleavage (PTC), is mediated by sequence tracts located downstream of the poly(A) site which appear to promote heterogeneous cleavage of the nascent transcript. The second event, in which pol II disengages from the DNA template, requires that polymerase has transcribed both a PTC sequence tract and a functional poly(A) site (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:1000, Immunocytochemistry 1:50, flow cytometry / FACS analysis 1:100 |
| Species Summary: | Hu |
| Alternate Names: | Anti-RNA polymerase II subunit B1 antibody, Anti-DNA-directed RNA polymerase II subunit A antibody, Anti-DNA-directed RNA polymerase III largest subunit antibody |
| Package Size: | 0.1 ml |
| General References: | 1. Bregman DB, et al. Proc Natl Acad 93(21):11586-90, 1996 2. Dye MJ, et al. Cell 105(5):669-81, 2001 |
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