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| Catalog Code: | NB100-79960 |
| Product Name: | GOS-28 Antibody |
| Product Description: | Rabbit Monoclonal anti-GOS-28 (EP1768Y) |
| Clone: | EP1768Y |
| Clonality: | Monoclonal |
| ListPrice: | 320 |
| Reference Sequence: | Q95249 |
| Immunogen: | A synthetic peptide corresponding to residues near the N-terminus of human GOS-28 was used as an immunogen. |
| Specificity: | A synthetic peptide corresponding to residues near the N-terminus of human GOS-28 was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, Rat |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, immunohistochemistry, Immunocytochemistry, flow cytometry / FACS analysis, immunoprecipitation |
| Background: | In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and delivers them after covalent modification to their destination in the cell. Although little is known about membrane-tubule-mediated transport, the molecular mechanism for vesicle-mediated transport is quite well understood, occurring through docking of SNAREs on the vesicle with those on the target membrane. (1). Transport of proteins along the exocytotic pathway is primarily achieved by vesicular intermediates. Two proteins, Golgi SNAREs of 27 kDa, GS27, and of 28 kDa, GS28 are important trafficking membrane proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments (2). In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:250-500, immunohistochemistry 1:100-250, Immunocytochemistry 1:50-100, immunoprecipitation 1:60, flow cytometry / FACS analysis 1:30 |
| Species Summary: | Hu, Mu, Rt |
| Package Size: | 0.1 ml |
| General References: | 1. Bui TD, et al. Genomics 57(2):285-8, 1999 2. Lowe SL, et al. Nature 389(6653):881-4, 1997 3. Nagahama M, et al. J Cell Biol 133(3):507-16 |
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