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| Catalog Code: | NB100-79945 |
| Product Name: | FAK Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-FAK Phospho (EP2160Y) [Tyr397] |
| Clone: | EP2160Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Reference Sequence: | Q05397 |
| Immunogen: | A phospho-specific peptide corresponding to residues surrounding Tyrosine 397 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y397. |
| Specificity: | A phospho-specific peptide corresponding to residues surrounding Tyrosine 397 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y397. |
| Cross Reactivity: | This antibody is reactive against Human, Rat |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot |
| Background: | Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:1000-2000 |
| Species Summary: | Hu, Rt(-) |
| Alternate Names: | Anti-FADK 1 antibody, Anti-pp125FAK antibody, Anti-Protein-tyrosine kinase 2 antibody |
| Package Size: | 0.1 ml |
| Phosphorylated: | Tyr397 |
| General References: | 1. Schaller, M.D. Biochim. Biophys. Acta 1540, 1-21, 2001. 2. Schaller, M.D., et al; .PNAS. 89:5192-5196, 1992. 3. Salazar, E.P, et al. J. Biol. Chem. 274:28371-28378, 1999 4. Tremblay L., et al. Int. J. Cancer, 68: 164-171, 1996 |
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