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| Catalog Code: | NB100-79941 |
| Product Name: | Ezh 1/2 Antibody |
| Product Description: | Rabbit Monoclonal anti-Ezh 1/2 (EP1408Y) |
| Clone: | EP1408Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Reference Sequence: | Q15910 |
| Immunogen: | A synthetic peptide corresponding to residues near the C-terminus of human Ezh 1/2 was used as an immunogen. |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human Ezh 1/2 was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against Human |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, Immunocytochemistry, flow cytometry / FACS analysis, immunoprecipitation |
| Background: | Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression.(2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:500, Immunocytochemistry 1:50-100, immunoprecipitation 1:20, flow cytometry / FACS analysis 1:30 |
| Species Summary: | Hu |
| Alternate Names: | Anti-ENX-1 antibody |
| Package Size: | 0.1 ml |
| General References: | 1. Hobert O, et al. Mol Cell Biol. 16(6):3066-73, 1996. 2. Cha TL, et al. Science 310(5746):306-10, 2005. 3. Su IH, et al. Nat Immunol 4(2):124-31, 2003. |
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