| request information: |
|
| Catalog Code: | NB100-79938 |
| Product Name: | eIF4E Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-eIF4E Phospho (EP2151Y) [Ser209] |
| Clone: | EP2151Y |
| Clonality: | Monoclonal |
| ListPrice: | 320 |
| Reference Sequence: | P06730 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen. |
| Specificity: | A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, Rat |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, immunohistochemistry, Immunocytochemistry, immunoprecipitation |
| Background: | eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:5000-1:10000, immunohistochemistry 1:100-250, Immunocytochemistry 1:500, immunoprecipitation 1:60 |
| Species Summary: | Hu, Mu, Rt |
| Alternate Names: | Anti-eIF4E antibody, Anti-eIF-4E antibody, Anti-mRNA cap-binding protein antibody, Anti-eIF-4F 25 kDa subunit antibody |
| Package Size: | 0.1 ml |
| Phosphorylated: | Ser209 |
| General References: | 1. Rychlik, et al Proc. Nat. Acad. Sci. 84: 945-949, 1987. 2. Rhoads, R. E., Joshi-Barve, S., and Rinker-Schaeffer, C.(1993) Prog. Nucleic Acids Res. Mol. Biol.46, 183-219 3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3 3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3 4. Jone et al. Molec. Cell. Biol. 16: 4754-4764, 1996. 5. Wendel et al. Nature 428: 332-337, 2004. |
order or
more information: | company product webpage |
|