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| Catalog Code: | NB100-79934 |
| Product Name: | eEF2 Antibody |
| Product Description: | Rabbit Polyclonal anti-eEF2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Reference Sequence: | P13639 |
| Immunogen: | Specific to synthetic peptide corresponding to residues in the C-terminus of human eEF2 protein. |
| Epitope: | (C-term) |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, Rat |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera |
| Uses: | This antibody is useful for: Western Blot, immunohistochemistry, Immunocytochemistry, flow cytometry / FACS analysis, immunoprecipitation |
| Background: | The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated. Phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:50000, immunohistochemistry 1:100-1:25, Immunocytochemistry 1:50-1:100, immunoprecipitation 1:50, flow cytometry / FACS analysis 1:20 |
| Species Summary: | Hu, Mu, Rt |
| Alternate Names: | Anti-EF-2 antibody |
| Package Size: | 0.1 ml |
| General References: | 1. Rapp, G.; Klaudiny, J.; Hagendorff, G.; Luck, M. R.; Scheit, K. H. : Biol. Chem. Hoppe-Seyler 370: 1071-1075, 1989. 2. Carlberg, U., Nilsson, A. and Nygard, O. (1990) Eur. J. Biochem. 191, 639-645 3. Price, N.T., Redpath, N.T., Severinov, K.V., Campbell, D.G., Russell, J.M. and Proud, C.G. (1991) FEBS Lett. 282, 253-258 4. Ryazanov, A.G., Natapov, P.G., Shestakova, E.A., Severin, F.F. and Spirin, A.S. (1988) Biochimie 70, 619-626 |
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