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| Catalog Code: | NB100-79907 |
| Product Name: | Btk Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Btk Phospho (EP420Y) [Tyr223] |
| Clone: | EP420Y |
| Clonality: | Monoclonal |
| ListPrice: | 320 |
| Reference Sequence: | Q06187 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223. |
| Specificity: | A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223. |
| Cross Reactivity: | This antibody is reactive against Human |
| Packaging: | 0.1 ml Rabbit ascites |
| Uses: | This antibody is useful for: Western Blot, immunoprecipitation |
| Background: | Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3) Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | Western Blot 1:100000-500000, immunoprecipitation 1:40 |
| Species Summary: | Hu |
| Alternate Names: | Anti-Bruton tyrosine kinase antibody, Anti-Agammaglobulinaemia tyrosine kinase antibody, Anti-ATK antibody, Anti-B-cell progenitor kinase antibody, Anti-BPK antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% Sodium Azide |
| Phosphorylated: | Tyr223 |
| General References: | 1. Tsukada, S., et al (1993) Cell 72, 279-290 2. Rawlings, DJ et al. Immunol. 7, 237-246 3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656 4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304-5311. |
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