| CatalogCode: | NB100-794 |
| ProductName: | XAB2 Antibody |
| Product Description: | Goat Polyclonal anti-XAB2 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide SVPAAVFGSLKED, representing the C terminus of the human protein (residues 843-855) of Human XAB2. |
| CrossReactivity: | Reacts with Human. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Dog and Zebrafish (83% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used in Peptide ELISA and Western Blot. In western blot a 100 kDa band is seen A431 lysate.Not tested in other applications.Recommended Starting Dilutions*: ELISA: 1/8000WB: 0.3 - 1.0 ug/ml. *Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear, detected in the splicing complex carrying pre-mRNA. |
| Control: | A431 lysate. |
| Background: | Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Homolog of yeast Tim50 antibody, anti-TIM50 antibody, anti-Tim50 like protein antibody, anti-TIM50L antibody, anti-Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody, anti-DKFZp762C1015 antibody, anti-HCNP antibody, anti-HCRN antibody, anti-NTC90 antibody, anti-SYF1 antibody |
| ProteinTarget: | XAB2 antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | b>Nakatsu Y et al. XAB2, a novel tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. J Biol Chem 275:34931-7 (2000). |
| Gene_Id: | 56949 |
| Omim_ID: | NB100-218 |