| CatalogCode: | NB100-78569 |
| ProductName: | ER alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-ER alpha |
| Clonality: | Polyclonal |
| Immunogen: | Modified peptide |
| CrossReactivity: | Human. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated. |
| Control: | Widely expressed in different tissue types. High expression of ERs is observed in a large population |
| Background: | ER alpha (Estrogen receptor alpha) belongs to the nuclear hormone receptor family, NR3 subfamily. Contains an N-terminal domain with a hormone-independent transcriptional activation function (AF-1), a central DNA-binding domain, and a C-terminal ligand-binding domain with a hormone-dependent transcriptional activation function (AF-2). Predicted molecular weight approximately 66 kD. Estrogen receptor has two subtypes, ER alpha and ER beta. Liganded ER alpha and ER beta binds as a homodimer or as a hetrodimer with an estrogen responsive element and regulate the target gene transcription. Estrogen receptors mediate most of the biological effects of estrogen in mammary and uterine epithelial cells. High expression of ERs is observed in a large population of breast tumors. It has been reported AKT1, AKT2, p90RSK3, or p90RSK1 are capable to phosphorylate ser-167 of the human ER and Ser-167 may play a role in controlling AF-1 function. The Poly6365 antibody recognizes human phospho-ER alpha (Ser 167) and has been shown to be useful for immunoflorence staining. This antibody does not detect ER alpha phosphorylated at other sites. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | 325 |
| AppSummary: | IF |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ER antibody, anti-ESR antibody, anti-Estrogen receptor 1 antibody |
| ProteinTarget: | ER alpha |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Walter, P., et al. 1985. Proc. Nat. Acad. Sci. 82: 7889 2. Joel, P.B., 1998. Mol Cell Biol. 18: 1978 3. Campbell, R. A., et al. 2001. J. Biol. Chem. 276: 9817 |