| CatalogCode: | | NB100-78550 |
| ProductName: | | Caspase-9 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Caspase-9 |
| Clonality: | | Polyclonal |
| Immunogen: | | peptide mapping to an internal domain within the p10 subunit of human caspase-9 |
| CrossReactivity: | | Human, Mouse, Rat. |
| Packaging: | | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | | Each lot of this antibody is quality control tested by Western blotting. nbsp Western blotting suggested working dilution(s): Use nbsp10 microliter per 5 ml antibody dilution buffer for each mini-gel. nbsp It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | | Cytoplasm, when activated mitochondrial |
| Background: | | Caspase-9 is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase-9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase-9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase-3 and Caspase-6. Caspase-9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase-3, possibly Caspase-8 and Caspase-10 into large p37 and small p10 subunits. Caspase-9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The Poly6342 antibody has been shown to be useful for western blotting of nbsp human Human, Mouse and rat caspase-9 (p10) and caspase-9 precursor. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-Caspase-9 precursor antibody, anti-Casp9 antibody, anti-ICE-like apoptotic protease 6 (ICE-LAP6) antibody, anti-Apoptotic protease Mch-6 antibody, anti-Apoptotic protease activating factor 3 (Apaf-3) antibody |
| ProteinTarget: | | Caspase-9 |
| PackageSize: | | 0.2 ml |
| GeneralRef: | | 1. Srinivasula, S., et al., 1996. J. Biol. Chem. 271:27099. 2. Hu, Y., et al., 1998. PNAS 95:4386. 3. Sitailo, L., et al., 2002. J. Biol. Chem. 277:19346. 4. Potokar, M., et al., 2003. FEBS Lett. 544:153. |
| more information: | | company product webpage |
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