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| Catalog Code: | NB100-78549 |
| Product Name: | Caspase-3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase-3 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Peptide mapping to the amino terminal domain of human caspase-3 prescursor |
| Cross Reactivity: | Human. |
| Packaging: | 0.5 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. nbsp Western blotting suggested working dilution(s): Use 25 microliter per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and beta-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation nbsp and immunohistochemistry of nbsp human Human caspase-3 precursor. |
| Storage: | Store at 4C. Do not freeze. DO NOT FREEZE. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Caspase 3 preproprotein antibody, anti-Casp3 antibody, anti-cpp32 antibody, anti-Apopain antibody, anti-Yama antibody, anti-PARP cleavage protease antibody, anti-SREBP cleavage activity 1 antibody |
| Package Size: | 0.5 ml |
| General References: | 1. Goldberg, Y., et al., 1996. Nat. Genet. 13:442. 2. Fernandes-Alnemri, T., et al., 1996. PNAS 93:7464. 3. Slee, E., et al., 2001. J. Biol. Chem. 276:7320. 4. Woo, M., et al., 2003. Nat. Immunol. 4:1016. |
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