| CatalogCode: | NB100-78547 |
| ProductName: | BCL-X Antibody |
| Product Description: | Mouse Monoclonal anti-BCL-X (2H12) |
| Clone: | 2H12 |
| Clonality: | Monoclonal |
| Immunogen: | Amino acids: 3-14 common to human and mouse Bcl-X |
| CrossReactivity: | Human, Mouse, Rat, Porcine. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. nbspIt is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developi |
| Background: | Bcl-X is one of the members of Bcl-2 family proteins, and exists in several isoforms generated by alternative splicing and plays a critical role in the control of apoptosis. The large isoform Bcl-XL (30KD) contains the highly conserved BH1 and BH2 amino acid domains and protects cells against apoptosis, whereas the short isoform, Bcl-XS (18KD) which deletes the conserved BH1/BH2 domain by alternative mRNA splicing, promotes apoptosis by interacting with Bcl-XL and Bcl-2. Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. Clone 2H12 has been shown to be useful for western blotting and immunoprecipitation, immunohistochemistry, and immunofluorescence of the Human, Mouse and Rat Bcl-XL and Bcl-XS proteins. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host_Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0 |
| ListPrice: | 285 |
| AppSummary: | ICC, FACS, IHC, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt, Po |
| ALTnames: | anti-Apoptosis regulator Bcl-X antibody, anti-Bcl-2-like 1 protein antibody |
| ProteinTarget: | BCL-X |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Boise, L. H., et al. 1993, Cell 74: 597 2. W Fang., et al. 1994. J. Immunol. 153: 4388 3. Deverman B., et al. 2002. Cell 111:51 |
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