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| Catalog Code: | NB100-78546 |
| Product Name: | Bax Antibody |
| Product Description: | Mouse Monoclonal anti-Bax (6A7) |
| Clone: | 6A7 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Amino acid:12-24 common to human, mouse, and rat Bax protein |
| Cross Reactivity: | Human, Mouse, Rat, Monkey. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. nbspIt is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Bax is a 21 kD pro-apoptotic protien known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 6A7 has been shown to be useful for western blotting and immunoprecipitation of the Human, Mouse, Rat and Monkey Bax protein. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0 |
| Application Summary: | immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, Mk |
| Alternate Names: | anti-BCL2 associated X protein antibody, anti-apoptosis regulator Bax antibody |
| Package Size: | 0.1 mg |
| General References: | 1. LeBlanc, H., et al. 2002. Nat. Med. 8:274.2. Marzo, I., et al. 1998. Science 281:2027.3. Miyashita, T, et al. 1995. Cell 80:293.4. Oltvai, Z. N., et al. 1993. Cell 74:609. |
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