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| Catalog Code: | NB100-78543 |
| Product Name: | Bcl-2 Antibody |
| Product Description: | Mouse Monoclonal anti-Bcl-2 (BCL/10C4) |
| Clone: | BCL/10C4 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | N-terminal, Amino acid residues 61-76 of mouse Bcl-2 |
| Cross Reactivity: | Mouse, Rat. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist alpha=25 kD; beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0 |
| Application Summary: | Immunocytochemistry, flow cytometry / FACS analysis, immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Mu, Rt |
| Alternate Names: | anti-B-cell leukemia 2 antibody |
| Package Size: | 0.1 mg |
| General References: | 1. Tsujimoto, Y., et al., 1986 PNAS 83:5214. 2. Yang, E., et al., 1995. Cell 80:285. 3. Huang, Z., et al., 2000. Oncogene 19:6627. 4. Deng, X., et al., 2003. Blood. 102:3179. |
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