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| Catalog Code: | NB100-78535 |
| Product Name: | B-Raf Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-B-Raf [Thr598/Ser601] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Modified peptide |
| Cross Reactivity: | Human. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Immunofluorescence staining. For immunofluorescence microscopy, suggested working dilution(s): Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The Raf proteins are a family of serine/threonine-specific kinases that serve as a central intermediate in transmitting extracellular signals to the mitogen-activated protein kinase cascade, which controls cell growth, differentiation and survival. Three isoforms of Raf proteins have been found in mammalian cells: Raf-1, A-Raf and B-Raf. The B-raf involved in signal transduction from the membrane to the nucleus. It has been reported that T598 and S601 are the major phosphorylation sites of B-Raf in response to oncogenic Ras, and phosphorylation of these two residues is required for full activation of B-Raf. Mutations in the B-Raf gene have been reported in a number of human cancers, including malignant melanoma, thyroid cancer, and colorectal carcinoma. The poly6327 antibody recognizes human phosphorylated B-Raf (Thr598/Ser601) and has been shown to be useful for immunoflorence staining. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | immunofluorescence |
| Species Summary: | Hu |
| Alternate Names: | anti-B-Raf proto-oncogene serine/threonine-protein kinase antibody, anti-BRAF antibody, anti-p94 antibody |
| Package Size: | 0.1 ml |
| Phosphorylated: | Thr598/Ser601 |
| General References: | 1. Avruch J., et al. 1994. Trends Biochem Sci. 19:279.2. Brose, M. S., et al. 2002. Cancer Res. 62:6997.3. Zhang, B-H., et al. 2000. EMBO 19:5429. |
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