| request information: | |
| Catalog Code: | NB100-78523 |
| Product Name: | GAPDH Antibody |
| Product Description: | Rabbit Polyclonal anti-GAPDH |
| Clonality: | Polyclonal |
| ListPrice: | 340 |
| Immunogen: | Peptide-KLH (C-terminal) |
| Cross Reactivity: | Human, Mouse, Rat, Rabbit. |
| Packaging: | 0.5 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 mul antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | This rabbit polyclonal antibody recognizes human, mouse, rat, and rabbit GAPDH also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 37 kDa. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. The poly6314 antibody has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.1 |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, Rb |
| Alternate Names: | anti-Glyceraldehyde 3 phosphate dehydrogenase antibody |
| Package Size: | 0.5 ml |
| General References: | 1. Ercolani L., et al. 1988. J. Biol. Chem. 263:15335.2. Meyer-Siegler K., et al. 1991. Proc. Natl. Acad. Sci. U.S.A. 88:8460.3. Bae, B.I., et al. 2006. Proc. Natl. Acad. Sci. U.S.A. 103:3405. |
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