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| Catalog Code: | NB100-78474 |
| Product Name: | TPX2 Antibody |
| Product Description: | Mouse Monoclonal anti-TPX2 (18D5), conjugated to Biotin |
| Clone: | 18D5 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Amino Acid: 1-220 of human TPX2 |
| Cross Reactivity: | Human. |
| Packaging: | 0.25 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, alpha-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0 |
| Application Summary: | immunohistochemistry immunofluorescence, immunofluorescence , Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Targeting protein for Xklp1 antibody, anti-restricted expression proliferation associated protein p100 antibody, anti-differentially expressed in cancer and non-cancerous lung cells 2 (DIL2) antibody |
| Package Size: | 0.25 mg |
| Conjugate: | Biotin |
| General References: | 1. Gruss, O. J., et al., 2002. Nature Cell Biol. 4:871.2. Heidebrecht, H. J., et al., 1997. Blood 90:226.3. Kufer, T.A., et al., 2002. J. Cell. Biol. 158:617. |
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