| CatalogCode: | NB100-78472 |
| ProductName: | Tubulin-alpha Antibody |
| Product Description: | Mouse Monoclonal anti-Tubulin-alpha (10D8), conjugated to Biotin |
| Clone: | 10D8 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length human alpha-tubulin |
| CrossReactivity: | Human, Mouse, Rat, and all specices. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescent staining applications, a concentration range of 1-4 mug/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Ubiquitous |
| Background: | Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgM, kappa |
| Host_Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0 |
| ListPrice: | 325 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Hu, Mu, Rt, Ma |
| ALTnames: | anti-alpha tubulin antibody, anti-Tubulin antibody, anti-alpha-ubiquitous chain antibody, anti-alpha-tubulin antibody |
| ProteinTarget: | Tubulin-alpha |
| PackageSize: | 0.1 mg |
| Conjugate: | Biotin |
| GeneralRef: | 1. Cowan, N.J., et al., 1983. Mol. Cell. Biol. 3:1738.2. Miller, F.D., et al., 1987. J. Cell. Biol. 105:3065.3. Wilde, C. D., et al., 1982. Proc. Natl. Acad. Sci. USA 79:96. |
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