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| Catalog Code: | NB100-78465 |
| Product Name: | PLK-1 Antibody |
| Product Description: | Mouse Monoclonal anti-PLK-1 (3F8), conjugated to Biotin |
| Clone: | 3F8 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Amino Acid: 300-603 of human PLK-1 |
| Cross Reactivity: | Human, Mouse. |
| Packaging: | 0.25 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0 |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-Serine/Threonine protein kinase PLK antibody, anti-Polo-like kinase (PLK) antibody, anti-Serine-threonine protein kinase 13 antibody, anti-plk1 antibody |
| Package Size: | 0.25 mg |
| Conjugate: | Biotin |
| General References: | 1. Hamanaka, R., et al., 1994. Cell Growth Differ. 5:249. 2. Lake, R. J., et al., 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich, U., et al., 1994. PNAS 91:1736. |
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