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| Catalog Code: | NB100-78457 |
| Product Name: | cdc2 Antibody |
| Product Description: | Mouse Monoclonal anti-cdc2 (POH-1) |
| Clone: | POH-1 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant human cdc2 protein |
| Cross Reactivity: | Human, Monkey, Cattle (Bovine). Does not react with mouse, rat, Xenopus, Drosophila. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry-paraffin. For immunofluorescence microscopy: Use a dilution range of 1-4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M; cyclin dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally in eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine, and tyrosine; tyrosine phosphorylation is subject to cell cycle regulation. Cdc2 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. The POH-1 antibody recognizes human, monkey and bovine cdc2 and is useful for Western blotting. The POH-1 antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (formalin-fixed, paraffin embedded), and immunofluorescence. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0 |
| Application Summary: | immunofluorescence , immunoprecipitation , Western Blot , Immunohistochemistry-Paraffin |
| Species Summary: | Hu, Mk, Bv |
| Alternate Names: | anti-Cell division cycle 2 antibody, anti-G1 to S and G2 to M; cyclin dependent kinase 1 antibody, anti-p34 kinase antibody, anti-CDK1 antibody |
| Package Size: | 0.1 mg |
| General References: | 1. Draetta, G., et al., 1988. Nature 336:738. 2. Lee, M.G., et al., 1988. Nature 333:676. 3. Tan, M., et al., 2002. Molec.Cell 9:993. |
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