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| Catalog Code: | NB100-78449 |
| Product Name: | CPF Antibody |
| Product Description: | Rabbit Polyclonal anti-CPF |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Peptide corresponding to amino acids 53-123 of human CPF, member 2, variant 2 |
| Cross Reactivity: | Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | This polyclonal antibody recognizes the orphan nuclear receptor CPF also known as nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CYP7A promoter binding factor, and LRH1, is a 54 kD nuclear receptor protein. CPF is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. CPF contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains and binds to DNA as a monomer. CPF is known to regulate transcription of the liver enzyme cholesterol 7-alpha-hydrolase that converts cholesterol to bile acids and to activate the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. CPF interacts with multiprotein bridging actor 1 and the orphan nuclear receptor SHP and has been suggested to regulate pancreas-specific gene expression and play a role in embryonic development. There at least 3 alternatively spliced isoforms of CPF; isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells such as HepG2. The Poly6261 recognizes human CPF (amino acids 53-123) and has been shown to be useful for Western blotting. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Nuclear receptor subfamily 5 group A member 2 antibody, anti-orphan nuclear receptor NR5A2 antibody, anti-HB1F antibody, anti-fetoprotein transcription factor antibody, anti-FTF antibody, anti-CYP7A promoter binding factor antibody, anti-LRH1 antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Li, M., et al., 1998. J. Biol. Chem. 273:29022. 2. Nitta, M., et al., 1999. PNAS 96:6660. 3. Lee, Y. K., et al., 2002. J. Biol. Chem. 277:2463. 4. Zhang, C. K., et al., 2001. Gene 273:239. |
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