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| Catalog Code: | NB100-78442 |
| Product Name: | SLP-76 Antibody |
| Product Description: | Mouse Monoclonal anti-SLP-76 (H76), conjugated to FITC |
| Clone: | H76 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Human. |
| Packaging: | 25 tests Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microliter per million cells or 20 microliter per 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu |
| Alternate Names: | anti-Lymphocyte cytoplasmic protein 2 (LCP2) antibody, anti-SH2 domain containing leukocyte 76kD protein antibody, anti-SLP 76 antibody |
| Package Size: | 25 tests |
| Conjugate: | FITC |
| General References: | 1. Jackman, K.J., et al. 1995. J. Biol. Chem. 270:7029.2. Wardenberg, J.B., et al. 1996. J. Biol. Chem. 271:19641.3. Tuosto, L., et al. 1996. J. Exp. Med. 184:1161.4. Motto, D.G., et al. 1996. J. Exp. Med. 183:1937. |
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