| CatalogCode: | NB100-78441 |
| ProductName: | SLP-76 Antibody |
| Product Description: | Mouse Monoclonal anti-SLP-76 (H76) |
| Clone: | H76 |
| Clonality: | Monoclonal |
| Immunogen: | ammino acids 216-434 of human SLP-76 |
| CrossReactivity: | Human, Mouse. |
| Packaging: | 0.2 ml Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung. |
| Background: | SLP-76 is a human Src homology domain-containing leukocyte protein. nbspThis cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host_Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | 325 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Lymphocyte cytoplasmic protein 2 (LCP2) antibody, anti-SH2 domain containing leukocyte 76kD protein antibody, anti-SLP 76 antibody |
| ProteinTarget: | SLP-76 |
| PackageSize: | 0.2 ml |
| GeneralRef: | 1. Jackman, K.J., et al. 1995. J. Biol. Chem. 270:7029.2. Wardenberg, J.B., et al. 1996. J. Biol. Chem. 271:19641.3. Tuosto, L., et al. 1996. J. Exp. Med. 184:1161.4. Motto, D.G., et al. 1996. J. Exp. Med. 183:1937. |
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