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| Catalog Code: | NB100-78432 |
| Product Name: | NEK9 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-NEK9 [Thr210] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Partial recombinant protein, responding to the middle region of NEK-9 |
| Cross Reactivity: | Reacts with human NEK9. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | NEK9 is a 107 kD member of the serine/threonine-protein kinase family, NIMA subfamily containing a coiled-coil domain, and RCC1 repeats. This cytoplasmic protein forms dimers and is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation, Nek9 is activated during mitosis by intramolecular autophosphorylation at Thr210. Nek9 has been shown to interact with Ran GTPase (greater affinity for Ran-GDP over Ran-GTP), NEK6, NEK7, BICD2, histone H3, myelin basic protein, and beta-casein. The Poly6241 antibody recognizes human NEK9 and has been shown to be useful for Western blotting, immunohistochemistry, and immunoprecipitation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Serine/Threonine-protein kinase Nek9 antibody, anti-NimA-related protein kinase 9 antibody |
| Package Size: | 0.2 ml |
| Phosphorylated: | Thr210 |
| General References: | 1. Holland, P., et al., 2002. J. Biol. Chem. 277:16229. 2. Roig, J., et al., 2002. Genes Dev. 16:1640. 3. Belham, C., et al., 2003. J. Biol. Chem. 278:34897. |
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