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| Catalog Code: | NB100-78424 |
| Product Name: | Caspase-3 Antibody |
| Product Description: | Mouse Monoclonal anti-Caspase-3 (4-1-18) |
| Clone: | 4-1-18 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant full length human protein |
| Cross Reactivity: | Human, recognizes 32 kD pro-caspase-3 and 17 kD cleaved caspase-3. |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Caspase 3 (also known as cpp32, Apopain, Yama, and PARP cleavage protease) is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. This caspase cleaves/activates SREBPs, Caspase 6, Caspase 7, and Caspase 9. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Active heterodimers form between the large p17 subunit and small p11 subunit of Caspase 7 and vice versa. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and beta-catenin. The 4-1-18 monoclonal antibody has been shown to be useful for Western blotting of human caspase 3 (32 kD pro-caspase and 17 kD cleaved caspase). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2 |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Caspase 3 preproprotein antibody, anti-Casp3 antibody, anti-cpp32 antibody, anti-Apopain antibody, anti-Yama antibody, anti-PARP cleavage protease antibody, anti-SREBP cleavage activity 1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | Sodium Azide |
| General References: | 1. Goldberg, Y., et al., 1996. Nat. Genet. 13:442. 2. Fernandes-Alnemri, T., et al., 1996. PNAS 93:7464. 3. Slee, E., et al., 2001. J. Biol. Chem. 276:7320. 4. Woo, M., et al., 2003. Nat. Immunol. 4:1016. |
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