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| Catalog Code: | NB100-78420 |
| Product Name: | beta actin Antibody |
| Product Description: | Rabbit Polyclonal anti-beta actin |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Peptide-KLH, NH2 terminus |
| Cross Reactivity: | Mouse, Rat, Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | beta-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human beta-actin. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-Actin antibody, anti-cytoplasmic 1 beta actin antibody, anti-beta-actin antibody, anti-b-actin antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Hanukoglu, I., et al., 1983. J. Mol. Biol. 163:673.2. Nakajima-Iijima, S., et al., 1985. Proc. Natl. Acad. Sci. 82:6133.3. Ponte, P., et al., 1984. Nucleic Acids Res. 12:1687. |
| Novus References: | 1. Biswas SK, Zhao Y, Nagalingam A, et al. PDGF- and Insulin/IGF-1-Specific Distinct Modes of Class IA PI 3-Kinase Activation in Normal Rat Retinas and RGC-5 Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 2008;49(8):3687-3698. 2. Tuli A, Sharma M, McIlhaney MM, et al. Amyloid Precursor-Like Protein 2 Increases the Endocytosis, Instability, and Turnover of the H2-Kd MHC Class I Molecule. J Immunol 2008;181(3):1978-1987. |
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