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| Catalog Code: | NB100-78419 |
| Product Name: | Caspase-8 Antibody |
| Product Description: | Mouse Monoclonal anti-Caspase-8 (4-1-20) |
| Clone: | 4-1-20 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant full length human protein |
| Cross Reactivity: | Human, 55 kD pro-caspase form. |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Caspase 8 (also known as ICE-like apoptotic protease 5, MORT1-associated CED-3 homolog (MACH), FADD-like ICE (FLICE), and apoptotic protease Mch-5) is a member of the peptidase family C14 that contains a death effector domain. Nine isoforms of this caspase have been reported, pro-Caspase 8 has a molecular weight of 55 kD. This caspase is found in the cytoplasm and, upon activation, in the mitochondria. Caspase 8 is the most upstream protease of caspase activation cascade, responsible for TNFRSF6/FAS/TRAIL-R mediated and TNFRSF1A induced cell death. This caspase cleaves/activates Caspase 3, Caspase 4, Caspase 6, Caspase 7, Caspase 9, Caspase 10, and Bid. Caspase 8 can be inhibited by the dominant negative isoform, ARC, and c-FLIP. Caspase 8 autocleaves into large p18 and small p10 subunits when activated by recruitment to the DISC complex at plasma membrane by FADD. This caspase interacts with FADD, CFLAR, and PEA15 proteins. Caspase 8 isoform 9 interacts at the ER with a complex containing BCAP31, BAP29, Bcl-2 and/or Bcl-2L1. Substrates for caspase 8 include androgen receptor, beta-catenin, gelsolin, and vimentin. The 4-1-20 monoclonal antibody has been shown to be useful for Western blotting of human caspase 8 (55 kD pro-caspase form). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Fin |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Caspase-8 precursor antibody, anti-Casp8 antibody, anti-ICE-like apoptotic protease 5 antibody, anti-MORT1-associated CED-3 homolog (MACH) antibody, anti-FADD-like ICE (FLICE) antibody, anti-Apoptotic protease Mch-5 antibody |
| Package Size: | 0.1 mg |
| General References: | 1. Boldin, M., et al., 1996. Cell. 85:803. 2. Breckenridge, D., et al., 2002. PNAS 99:4331. 3. Himeji, D., et al., 2002. Blood. 99:4070. 4. van Noesel, M., 2003. Cancer Lett. 197:165. |
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