| CatalogCode: | NB100-78418 |
| ProductName: | Caspase-9 Antibody |
| Product Description: | Mouse Monoclonal anti-Caspase-9 (96-2-22) |
| Clone: | 96-2-22 |
| Clonality: | Monoclonal |
| Immunogen: | N-terminal fragment of human caspase-9 |
| CrossReactivity: | Human, recognizes 46 kD pro-caspase-9 and 34 kD cleaved caspase-9. |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Cytoplasm, when activated mitochondrial |
| Background: | Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The 96-2-22 monoclonal antibody has been shown to be useful for Western blotting of human caspase 9 (46 kD pro-caspase 9 as well as the 34 kD cleaved caspase 9). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1, kappa |
| Host_Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Fin |
| ListPrice: | 285 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Caspase-9 precursor antibody, anti-Casp9 antibody, anti-ICE-like apoptotic protease 6 (ICE-LAP6) antibody, anti-Apoptotic protease Mch-6 antibody, anti-Apoptotic protease activating factor 3 (Apaf-3) antibody |
| ProteinTarget: | Caspase-9 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Srinivasula, S., et al., 1996. J. Biol. Chem. 271:27099. 2. Hu, Y., et al., 1998. PNAS 95:4386. 3. Sitailo, L., et al., 2002. J. Biol. Chem. 277:19346. 4. Potokar, M., et al., 2003. FEBS Lett. 544:153. |
order or
more information: | company product webpage |
| request information: |
|
|