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| Catalog Code: | NB100-78416 |
| Product Name: | EGFR Antibody |
| Product Description: | Rabbit Polyclonal anti-EGFR |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | peptide-KLH, C terminal |
| Cross Reactivity: | Mouse, Rat, Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The EGFR is a ubiquitously expressed 180 kD membrane tyrosine kinase that dimerizes upon binding EGF, transforming growth factor alpha, or other ligands. Dimerization initiates downstream signaling that elicits extensive phosphorylation of the receptor resulting in mitogenesis and protection against apoptotic stimuli. EGF receptor expression is amplified in many squamous carcinomas and in the bronchi of high-risk smokers. The receptor is internalized and degraded after ligand binding. EGFR extensively interacts with a number of proteins and is also reported to be a cellular receptor for cytomegalovirus. The Poly6213 antibody recognizes the human, mouse, and rat EGFR and has been shown to be useful for Western blotting. |
| Storage: | Upon receipt, store undiluted at -20 deg C. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2 |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-Epidermal growth factor receptor antibody, anti-EGF Receptor antibody, anti-ErbB-1 antibody, anti-HER1 antibody |
| Package Size: | 0.2 ml |
| Preservative: | Sodium Azide |
| General References: | 1. Carpenter, G. 1984. Cell 37:357.2. Ullrich, A., et al.,1984. Nature 309:418.3. Reynolds, F.H., Jr. et al., 1981. Nature 292:259.4. Sibilia, M., et al., 2000. Cell 102:211. |
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