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| Catalog Code: | NB100-78410 |
| Product Name: | Cdc25A Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdc25A [Ser17] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Modified peptide |
| Cross Reactivity: | Human, recognizes Ser17-phosphorylated Cdc25A. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Cdc25A (also know as M-phase inducer phosphatase 1 and dual specificity phosphatase Cdc25A) is a 65 kD MPI phosphatase containing a rhodanese domain. This nuclear protein functions as a dosage-dependent inducer in mitotic control required for the progression of cell cycle. Cdc25A activates Cdc2 by dephosphorylation, dephosphorylates CDK2 in complex with cyclin E. Cdc25A is timulated by cyclins B, downregulated by Chk1 and Cds1/Chk2 phosphorylation. This phosphatase has been shown to interact with Cdc2, cyclin B, Cdk2, Chk1, and Chk2. The Poly6207 antibody recognizes phosphorylated human Cdc25A (Ser17) and has been shown to be useful for Western blotting. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-M-phase inducer phosphatase 1 antibody, anti-Dual specificity phosphatase Cdc25A antibody |
| Package Size: | 0.05 ml |
| Phosphorylated: | Ser17 |
| General References: | 1. Galaktionov, K., et al., 1991. Cell. 67:1181. 2. Jinno, S., et al., 1994. EMBO J. 13:1549. 3. Ducruet, A., et al., 2003. J. Biol. Chem. 278:31838. 4. Sorensen, C., et al., 2003. Cancer Cell. 3:247. |
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