| CatalogCode: | | NB100-78371 |
| ProductName: | | 14-3-3 gamma/ delta Antibody |
| Product Description: | | Rabbit Polyclonal anti-14-3-3 gamma/ delta |
| Clonality: | | Polyclonal |
| Immunogen: | | Recombinant (partial), N-terminal |
| CrossReactivity: | | Mouse, Human. |
| Packaging: | | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | | Cytoplasm |
| Background: | | The 30 kD 14-3-3 cytoplasmic protein participates in signal transduction by binding to phosphoserine-containing proteins and activating protein kinase C. This protein has also been shown to activate tyrosine/ tryptophan hydroxylases in presence of Ca2+/calmodulin-dependent protein kinase II, regulate insulin sensitivity, inhibit tuberin function, participate in actin cytoskeletal alterations, regulate cell adhesion, and inhibit the pro-apoptotic protein Bad. This protein forms dimers and can be modified by acetylation and phosphorylation. The delta isoform of 14-3-3 is phosphorylated on serine-185; phosphorylation disrupts dimerization. The 14-3-3 protein has been shown to interact with MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, and Bad proteins. The Poly6148 antibody has been shown to be useful for Western blotting of the mouse and human 14-3-3 proten. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Mu, Hu |
| ALTnames: | | anti-Protein kinase C inhibitor protein-1 antibody, anti-Phospholipase A2 antibody, anti-Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody, anti-zeta polypeptide (YWHAZ) antibody |
| ProteinTarget: | | 14-3-3 gamma/ delta |
| PackageSize: | | 0.2 ml |
| GeneralRef: | | 1. Isobe, T., et al., 1992. FEBS Lett. 308:121. 2. Ogihara, T., et al., 1997. J. Biol. Chem. 272:25267. 3. Powell, D., et al., 2002. J. Biol. Chem. 277:21639. 4. Powell, D., et al., 2003. Mol. Cell Biol. 23:5376. |
| more information: | | company product webpage |
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